High Performance Liquid chromatography(HPLC)
Instrument Details
Make: Shimadzu
Model: 10AT vp
About Instrument:
HPLC instrument generally constitute of autosampler, pumps, and a detector. The autosampler injects specific volume of sample mixture into the mobile phase stream which carries it into the column. The pumps deliver the desired flow (isocratic or gradient) of the mobile phase through the column. The detector generates a signal proportional to the amount of sample component emerging from the column, hence allowing for quantitative analysis of the sample components. A digital microprocessor (Communication Bus Module) and user software (LC Solution) control the HPLC instrument and provide data analysis.
Working Principal
HPLC is a technique to separate mixtures of substances into their components on the basis of their molecular structure and molecular composition. This involves a stationary phase and a mobile phase. The mobile phase flows through the stationary phase and carries the components of the mixture with it. Sample components that display stronger interactions with the stationary phase will move more slowly through the column than components with weaker interactions. This difference in rates cause the separation of various components.
Detailed Specifications:
HPLC Pump (LC-10AT vp):
Flow rate range- 0.001ml /min to 9.0ml/min
Max. operating pressure- 400 kgf
Manual Injector:
Loop Size- 20 µl
Autosampler (SIL-20A):
Injection Volume Range- 0.1 to 100 µl
Column Oven (CTO-10A vp):
Temp. range- 4.0 to 80.0 ᵒC
UV/VIS Detector (SPD-10A vp):
wavelength range- 190 to 600 nm
RID Detector (RID-10A):
Refractive index range- 1 - 1.75 RIU
Communication Bus Module (CBM-20A):
System controller equipped with a data buffering function
Acts as an interface for connecting LC workstations, network-client computers, and analytical instruments via Ethernet
Software:
LC Solution
Hardware:
Computer system
Available Accessories:
- Mobile Phase Reservoir
- Filtration Unit
Applications
Detection of sucrose and Isoflavones (diadzein and genistein)
User Instruction
Users are required to bring following things by themselves
- Column
- Mobile Phase
- Syringe
- Syringe Filter (PVDF)
- PVDF membrane filter for filtration unit
- HPLC grade chemicals
- HPLC grade Water
| Contact : | Dr M.P Sharma |
| Principal Scientist (Microbiology) | |
| Phone Number: | 0731-2437971(O), 9926012261(M) |
| Email: | mahaveer620@gmail.com, dsrdirector@gmail.com |